Loss of polyubiquitin gene Ubb leads to metabolic and sleep abnormalities in mice.

ABSTRACT:

Aims

Ubiquitin performs essential roles in a myriad of signalling pathways required for cellular function and survival. Recently, we reported that disruption of the stress-inducible ubiquitin-encoding gene Ubb reduces ubiquitin content in the hypothalamus and leads to adult-onset obesity coupled with a loss of arcuate nucleus neurones and disrupted energy homeostasis in mice. Neuropeptides expressed in the hypothalamus control both metabolic and sleep behaviours. In order to demonstrate that the loss of Ubb results in broad hypothalamic abnormalities, we attempted to determine whether metabolic and sleep behaviours were altered in Ubb knockout mice.

Methods

Metabolic rate and energy expenditure were measured in a metabolic chamber, and sleep stage was monitored via electroen

SUBMITTER: Ryu KY 

PROVIDER: S-EPMC3029017 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature