Nogo-B is associated with cytoskeletal structures in human monocyte-derived macrophages.

ABSTRACT:

Background

The reticulon Nogo-B participates in cellular and immunological processes in murine macrophages. Since leukocytes are an essential part of the immune system in health and disease, we decided to investigate the expression of Nogo-A, Nogo-B and Nogo-C in different human immune cell subpopulations. Furthermore, we analyzed the localization of Nogo-B in human monocyte-derived macrophages by indirect immunofluorescence stainings to gain further insight into its possible function.

Findings

We describe an association of Nogo-B with cytoskeletal structures and the base of filopodia, but not with focal or podosomal adhesion sites of monocyte-derived macrophages. Nogo-B positive structures are partially co-localized with RhoA staining and Rac1 positive membrane ruffles. Fur

SUBMITTER: Schanda K 

PROVIDER: S-EPMC3029212 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature