Urokinase-type plasminogen activator inhibits efferocytosis of neutrophils.

ABSTRACT:

Rationale

Phagocytosis of apoptotic cells, also called efferocytosis, plays an essential role in the resolution of inflammation. Urokinase-type plasminogen activator (uPA) is a multifunctional protein that has been implicated in inflammatory conditions, including pneumonia and severe infection, which are often accompanied by the development of acute lung injury. However, the role of uPA in modulating efferocytosis of apoptotic neutrophils has not been defined.

Objectives

To characterize the role of uPA in regulation of efferocytosis and to delineate the underlying mechanisms involved in this process.

Methods

In vitro and in vivo phagocytosis, immunoprecipitation, and Western blotting assays.

Measurements and main results

The phagocytosis of apoptotic neutrophil

SUBMITTER: Yang Y 

PROVIDER: S-EPMC3029937 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature