Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma.

ABSTRACT:

Purpose

p27 localization and expression has prognostic and predictive value in cancer. Little is known regarding expression patterns of p27 in renal cell carcinoma (RCC) or how p27 participates in disease progression or response to therapy.

Experimental design

RCC-derived cell lines, primary tumors, and normal renal epithelial cells were analyzed for p27 expression, phosphorylation (T157 of the NLS), and subcellular localization. RCC-derived cell lines were treated with phosphatidylinositol 3-kinase (PI3K) and mammalian target of rapamycin (mTOR) inhibitors and effects on p27 localization were assessed. The potential contribution of cytoplasmic p27 to resistance to apoptosis was also evaluated.

Results

p27 was elevated in tumors compared with matched controls, and cyt

SUBMITTER: Kim J 

PROVIDER: S-EPMC3030253 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature