Orthovanadate and orthophosphate inhibit muscle force via two different pathways of the myosin ATPase cycle.

ABSTRACT: Measurements of the half-sarcomere stiffness during activation of skinned fibers from rabbit psoas (sarcomere length 2.5 μm, temperature 12°C) indicate that addition of 0.1 mM orthovanadate (Vi) to the solution produces a drop to ∼1/2 in number of force-generating myosin motors, proportional to the drop in steady isometric force (T(0)), an effect similar to that produced by the addition of 10 mM phosphate (Pi). However, in contrast to Pi, Vi does not change the rate of isometric force development. The depression of T(0) in a series of activations in presence of Vi is consistent with an apparent second-order rate constant of ∼1 × 10(3) M(-1) s(-1). The rate constant of T(0) recovery in a series of activations after removal of Vi is 3.5 × 10(-2) s(-1). These results, together with the findin

SUBMITTER: Caremani M 

PROVIDER: S-EPMC3030264 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature