Microarray-based identification of tomato microRNAs and time course analysis of their response to Cucumber mosaic virus infection.

ABSTRACT: A large number of plant microRNAs (miRNAs) are now documented in the miRBase, among which only 30 are for Solanum lycopersicum (tomato). Clearly, there is a far-reaching need to identify and profile the expression of miRNAs in this important crop under various physiological and pathological conditions. In this study, we used an in situ synthesized custom microarray of plant miRNAs to examine the expression and temporal presence of miRNAs in the leaves of tomato plants infected with Cucumber mosaic virus (CMV). Following computational sequence homology search and hairpin structure prediction, we identified three novel tomato miRNA precursor genes. Our results also show that, in accordance with the phenotype of the developing leaves, the tomato miRNAs are differentially expressed at differen

SUBMITTER: Lang QL 

PROVIDER: S-EPMC3030956 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature