Identification of a potent anti-IL-15 antibody with opposing mechanisms of action in vitro and in vivo.

ABSTRACT:

Background and purpose

Interleukin-15 (IL-15) is important in the activation and proliferation of lymphocytic cell populations and is implicated in inflammatory disease. We report the characterization of a novel monoclonal antibody DISC0280 which is specific for human IL-15.

Experimental approach

DISC0280 was characterized in a direct binding assay of IL-15 with IL-15 receptor α (IL-15Rα) and by its ability to alter IL-15 mediated proliferation of a range of cell lines (cytotoxic T lymphocyte line-2, M-07e, KIT225). A pharmacodynamic model injecting male C57/BL6 mice with IL-15 or IL-15/IL-15Rα, with or without DISC0280, and assessing changes in lymphocytic cell populations and serum cytokines was utilized.

Key results

DISC0280 inhibited the binding of IL-15 to IL-15R

SUBMITTER: Finch DK 

PROVIDER: S-EPMC3031067 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature