Essential metabolites of Mycobacterium tuberculosis and their mimics.

ABSTRACT: An organism requires a range of biomolecules for its growth. By definition, these are essential molecules which constitute the basic metabolic requirements of an organism. A small organic molecule with chemical similarity to that of an essential metabolite may bind to the enzyme that catalyzes its production and inhibit it, likely resulting in the stasis or death of the organism. Here, we report a high-throughput approach for identifying essential metabolites of an organism using genetic and biochemical approaches and then implement computational approaches to identify metabolite mimics. We generated and genotyped 5,126 Mycobacterium tuberculosis mutants and performed a statistical analysis to determine putative essential genes. The essential molecules of M. tuberculosis were classified as

SUBMITTER: Lamichhane G 

PROVIDER: S-EPMC3031304 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature