Detection of dynamic spatiotemporal response to periodic chemical stimulation in a Xenopus embryonic tissue.

ABSTRACT: Embryonic development is guided by a complex and integrated set of stimuli that results in collective system-wide organization that is both time and space regulated. These regulatory interactions result in the emergence of highly functional units, which are correlated to frequency-modulated stimulation profiles. We have determined the dynamic response of vertebrate embryonic tissues to highly controlled, time-varying localized chemical stimulation using a microfluidic system with feedback control. Our approach has enabled localized spatiotemporal manipulation of the steroid hormone dexamethasone (DEX) in Animal Cap (AC) tissues isolated from gastrulating Xenopus embryos. Using this approach we investigated cell-scale responses to precisely controlled stimulation by tracking the redistribut

SUBMITTER: Kim Y 

PROVIDER: S-EPMC3031512 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature