The development and characterization of a human mesothelioma in vitro 3D model to investigate immunotoxin therapy.

ABSTRACT:

Background

Tumor microenvironments present significant barriers to penetration by antibodies and immunoconjugates. Tumor microenvironments, however, are difficult to study in vitro. Cells cultured as monolayers exhibit less resistance to therapy than those grown in vivo and an alternative research model more representative of the in vivo tumor is more desirable. SS1P is an immunotoxin composed of the Fv portion of a mesothelin-specific antibody fused to a bacterial toxin that is presently undergoing clinical trials in mesothelioma.

Methodology/principal findings

Here, we examined how the tumor microenvironment affects the penetration and killing activity of SS1P in a new three-dimensional (3D) spheroid model cultured in vitro using the human mesothelioma cell line (NCI-H226)

SUBMITTER: Xiang X 

PROVIDER: S-EPMC3031536 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature