Identification of novel molecular targets for endometrial cancer using a drill-down LC-MS/MS approach with iTRAQ.

ABSTRACT:

Background

The number of patients with endometrial carcinoma (EmCa) with advanced stage or high histological grade is increasing and prognosis has not improved for over the last decade. There is an urgent need for the discovery of novel molecular targets for diagnosis, prognosis and treatment of EmCa, which will have the potential to improve the clinical strategy and outcome of this disease.

Methodology and results

We used a "drill-down" proteomics approach to facilitate the identification of novel molecular targets for diagnosis, prognosis and/or therapeutic intervention for EmCa. Based on peptide ions identified and their retention times in the first LC-MS/MS analysis, an exclusion list was generated for subsequent iterations. A total of 1529 proteins have been identified

SUBMITTER: Voisin SN 

PROVIDER: S-EPMC3031560 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature