Cdx2 levels modulate intestinal epithelium maturity and Paneth cell development.

ABSTRACT:

Background & aims

Caudal-related homeobox protein 2 (Cdx2) is an intestine-specific transcription factor that is important for intestinal development and intestine-specific gene expression. Cdx2 regulates intestinal cell-cell adhesion, proliferation, and the transcriptional activities of Wnt and β-catenin in cell culture systems. We generated transgenic mice that overexpress Cdx2 in the small intestinal and colonic epithelium to investigate the role of Cdx2 in differentiation and function of the intestinal epithelium.

Methods

We established 4 different lines of villin-Cdx2 transgenic mice. Intestines were collected from infant, 3-month old, and wild-type mice. Genes of interest and cell lineage markers were examined by polymerase chain reaction and immunohistochemistry.

R

SUBMITTER: Crissey MA 

PROVIDER: S-EPMC3031739 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature