Attenuated reovirus displays oncolysis with reduced host toxicity.

ABSTRACT:

Background

Although the naturally occurring reovirus causes only mild symptoms in humans, it shows considerable potential as an oncolytic agent because of its innate ability to target cancer cells. In immunocompromised hosts, however, wild-type reovirus can target healthy tissues, including heart, liver, pancreas and neural structures.

Methods

We characterized an attenuated form of reovirus (AV) derived from a persistently infected cell line through sequence analysis, as well as western blot and in vitro transcription and translation techniques. To examine its pathogenesis and oncolytic potential, AV reovirus was tested on healthy embryonic stem cells, various non-transformed and transformed cell lines, and in severe combined immunodeficiency (SCID) mice with tumour xenograf

SUBMITTER: Kim M 

PROVIDER: S-EPMC3031901 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature