P73 deficiency results in impaired self renewal and premature neuronal differentiation of mouse neural progenitors independently of p53.

ABSTRACT: The question of how neural progenitor cells maintain its self-renewal throughout life is a fundamental problem in cell biology with implications in cancer, aging and neurodegenerative diseases. In this work, we have analyzed the p73 function in embryonic neural progenitor cell biology using the neurosphere (NS)-assay and showed that p73-loss has a significant role in the maintenance of neurosphere-forming cells in the embryonic brain. A comparative study of NS from Trp73-/-, p53KO, p53KO;Trp73-/- and their wild-type counterparts demonstrated that p73 deficiency results in two independent, but related, phenotypes: a smaller NS size (related to the proliferation and survival of the neural-progenitors) and a decreased capacity to form NS (self-renewal). The former seems to be the result of p5

SUBMITTER: Gonzalez-Cano L 

PROVIDER: S-EPMC3032295 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature