An experimentally anchored map of transcriptional start sites in the model cyanobacterium Synechocystis sp. PCC6803.

ABSTRACT: There has been an increasing interest in cyanobacteria because these photosynthetic organisms convert solar energy into biomass and because of their potential for the production of biofuels. However, the exploitation of cyanobacteria for bioengineering requires knowledge of their transcriptional organization. Using differential RNA sequencing, we have established a genome-wide map of 3,527 transcriptional start sites (TSS) of the model organism Synechocystis sp. PCC6803. One-third of all TSS were located upstream of an annotated gene; another third were on the reverse complementary strand of 866 genes, suggesting massive antisense transcription. Orphan TSS located in intergenic regions led us to predict 314 noncoding RNAs (ncRNAs). Complementary microarray-based RNA profiling verified a hi

SUBMITTER: Mitschke J 

PROVIDER: S-EPMC3033270 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature