HCV subtype characterization among injection drug users: implication for a crucial role of Zhenjiang in HCV transmission in China.

ABSTRACT:

Background

HCV transmission is closely associated with drug-trafficking routes in China. However, the transmission route of HCV in Eastern China remains unclear. Here, we investigate the role of Zhenjiang city of Jiangsu province, an important transportation hub linking Shanghai with other regions of China, in HCV transmission.

Methodology/principal findings

A total of 141 whole blood samples were collected from injection drug users (IDUs) in Zhenjiang and then tested for HCV infection. Of them, 115 HCV positive plasmas were subjected to RNA extraction, RT-PCR amplification, and sequencing. The subtype characterization and the evolutionary origin of HCV strains circulating in Zhenjiang were determined using polygenetic or phylogeographic analyses. Seven HCV subtypes 1b, 2a,

SUBMITTER: Zhang C 

PROVIDER: S-EPMC3033423 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature