Proteomics and transcriptomics characterization of bile stress response in probiotic Lactobacillus rhamnosus GG.

ABSTRACT: Lactobacillus rhamnosus GG (GG) is a widely used and intensively studied probiotic bacterium. Although the health benefits of strain GG are well documented, the systematic exploration of mechanisms by which this strain exerts probiotic effects in the host has only recently been initiated. The ability to survive the harsh conditions of the gastrointestinal tract, including gastric juice containing bile salts, is one of the vital characteristics that enables a probiotic bacterium to transiently colonize the host. Here we used gene expression profiling at the transcriptome and proteome levels to investigate the cellular response of strain GG toward bile under defined bioreactor conditions. The analyses revealed that in response to growth of strain GG in the presence of 0.2% ox gall the transc

SUBMITTER: Koskenniemi K 

PROVIDER: S-EPMC3033674 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature