Modeling the effect of oxygen on the amperometric response of immobilized organoselenium-based S-nitrosothiol sensors.

ABSTRACT: Amperometric detection of S-nitrosothiols (RSNOs) at submicromolar levels in blood samples is of potential importance for monitoring endothelial function and other disease states that involve changes in physiological nitric oxide (NO) production. It is shown here that the elimination of dissolved oxygen from samples is critical when covalently attached diselenocystamine-based amperometric RSNO sensors are used for practical RSNO measurements. The newest generation of RSNO sensors utilizes an amperometric NO gas sensor with a thin organoselenium modified dialysis membrane mounted at the distal sensing tip. Sample RSNOs are catalytically reduced to NO within the dialysis membrane by the immobilized organoselenium species. In the presence of oxygen, the sensitivity of these sensors for measur

SUBMITTER: Hofler L 

PROVIDER: S-EPMC3033888 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature