Analysis of two novel midgut-specific promoters driving transgene expression in Anopheles stephensi mosquitoes.

ABSTRACT:

Background

Tissue-specific promoters controlling the expression of transgenes in Anopheles mosquitoes represent a valuable tool both for studying the interaction between these malaria vectors and the Plasmodium parasites they transmit and for novel malaria control strategies based on developing Plasmodium-refractory mosquitoes by expressing anti-parasitic genes. With this aim we have studied the promoter regions of two genes from the most important malaria vector, Anopheles gambiae, whose expression is strongly induced upon blood feeding.

Results

We analysed the A. gambiae Antryp1 and G12 genes, which we have shown to be midgut-specific and maximally expressed at 24 hours post-bloodmeal (PBM). Antryp1, required for bloodmeal digestion, encodes one member of a family of 7 try

SUBMITTER: Nolan T 

PROVIDER: S-EPMC3033896 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature