Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.

ABSTRACT: Studies in embryonic development have guided successful efforts to direct the differentiation of human embryonic and induced pluripotent stem cells (PSCs) into specific organ cell types in vitro. For example, human PSCs have been differentiated into monolayer cultures of liver hepatocytes and pancreatic endocrine cells that have therapeutic efficacy in animal models of liver disease and diabetes, respectively. However, the generation of complex three-dimensional organ tissues in vitro remains a major challenge for translational studies. Here we establish a robust and efficient process to direct the differentiation of human PSCs into intestinal tissue in vitro using a temporal series of growth factor manipulations to mimic embryonic intestinal development. This involved activin-induced defi

SUBMITTER: Spence JR 

PROVIDER: S-EPMC3033971 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature