Herpes simplex virus type 1 primary envelopment: UL34 protein modification and the US3-UL34 catalytic relationship.

ABSTRACT: The herpes simplex virus type 1 (HSV-1) US3 kinase is likely important for primary envelopment of progeny nucleocapsids since it localizes to the nuclear envelope of infected cells and largely determines the phosphorylation state and localization of the necessary primary envelopment factor, the UL34 protein. In HEp-2 cells, the production of infectious US3 null progeny is delayed and decreased relative to that of the parental strain, HSV-1(F). Furthermore, the US3 kinase affects the morphology of primary envelopment such that in its absence, UL34 protein-containing enveloped virions accumulate within membrane-bound vesicles. These vesicles are most often found along the interior periphery of the nucleus and may be derived from the inner nuclear membrane. Since the US3 and UL34 proteins com

SUBMITTER: Ryckman BJ 

PROVIDER: S-EPMC303423 | biostudies-literature | 2004 Jan

REPOSITORIES: biostudies-literature