Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells.

ABSTRACT:

Background

Cytotoxic cells of the immune system have evolved a lysosomal compartment to store and mobilize effector molecules. In T lymphocytes and NK cells, the death factor FasL is one of the characteristic marker proteins of these so-called secretory lysosomes, which combine properties of conventional lysosomes and exocytotic vesicles. Although these vesicles are crucial for immune effector function, their protein content in T cells has so far not been investigated in detail.

Results

In the present study, intact membranous vesicles were enriched from homogenates of polyclonally activated T cells and initially characterized by Western blotting and electron microscopic inspection. The vesicular fraction that contained the marker proteins of secretory lysosomes was subsequen

SUBMITTER: Schmidt H 

PROVIDER: S-EPMC3034720 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature