Novel domains in the hnRNP G/RBMX protein with distinct roles in RNA binding and targeting nascent transcripts.

ABSTRACT: The heterogenous nuclear ribonucleoprotein G (hnRNP G) controls the alternative splicing of several pre-mRNas. While hnRNP G displays an amino terminal RNA recognition motif (RRM), we find that this motif is paradoxically not implicated in the recruitment of hnRNP G to nascent transcripts in amphibian oocytes. In fact, a deletion analysis revealed that targeting of hnRNP G to active transcription units depends on another domain, centrally positioned, and consisting of residues 186-236. We show that this domain acts autonomously and thus is named NTD for nascent transcripts targeting domain. Furthermore, using an RNA probe previously characterized in vitro as an RNA that interacts specifically with hnRNP G, we demonstrate a new auxiliary RNA binding domain (RBD). It corresponds to a short r

SUBMITTER: Kanhoush R 

PROVIDER: S-EPMC3035123 | biostudies-literature | 2010 Jan-Feb

REPOSITORIES: biostudies-literature