Characterization of L1 retrotransposition with high-throughput dual-luciferase assays.

ABSTRACT: Recent studies employing genome-wide approaches have provided an unprecedented view of the scope of L1 activities on structural variations in the human genome, and further reinforced the role of L1s as one of the major driving forces behind human genome evolution. The rapid identification of novel L1 elements by these high-throughput approaches demands improved L1 functional assays. However, the existing assays use antibiotic selection markers or fluorescent proteins as reporters; neither is amenable to miniaturization. To increase assay sensitivity and throughput, we have developed a third generation assay by using dual-luciferase reporters, in which firefly luciferase is used as the retrotransposition indicator and Renilla luciferase is encoded on the same or separate plasmid for normali

SUBMITTER: Xie Y 

PROVIDER: S-EPMC3035435 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature