Glycomics analysis of mammalian heparan sulfates modified by the human extracellular sulfatase HSulf2.

ABSTRACT:

Background

The Sulfs are a family of endosulfatases that selectively modify the 6O-sulfation state of cell-surface heparan sulfate (HS) molecules. Sulfs serve as modulators of cell-signaling events because the changes they induce alter the cell surface co-receptor functions of HS chains. A variety of studies have been aimed at understanding how Sulfs modify HS structure, and many of these studies utilize Sulf knockout cell lines as the source for the HS used in the experiments. However, genetic manipulation of Sulfs has been shown to alter the expression levels of HS biosynthetic enzymes, and in these cases an assessment of the fine structural changes induced solely by Sulf enzymatic activity is not possible. Therefore, the present work aims to extend the understanding of substrate

SUBMITTER: Staples GO 

PROVIDER: S-EPMC3035651 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature