Short distance movement of genomic negative strands in a host and nonhost for Sugarcane mosaic virus (SCMV).

ABSTRACT:

Background

In order to obtain an initial and preliminary understanding of host and nonhost resistance in the initial step of potyvirus replication, both positive and negative Sugarcane mosaic virus (SCMV) strands where traced in inoculated and systemic leaves in host and nonhost resistant maize and sugarcane for one Mexican potyviral isolate (SCMV-VER1). Intermediary replication forms, such as the negative viral strand, seem to only move a short distance as surveyed by RT-PCR analysis and ELISA in different leaves. Virus purification was also done in leaves and stems.

Results

Susceptible maize plants allowed for viral SCMV replication, cell-to-cell, and long distance movement, as indicated by the presence of the coat protein along the plant. In the host resistant maize plant

SUBMITTER: Chaves-Bedoya G 

PROVIDER: S-EPMC3036626 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature