The RON2-AMA1 interaction is a critical step in moving junction-dependent invasion by apicomplexan parasites.

ABSTRACT: Obligate intracellular Apicomplexa parasites share a unique invasion mechanism involving a tight interaction between the host cell and the parasite surfaces called the moving junction (MJ). The MJ, which is the anchoring structure for the invasion process, is formed by secretion of a macromolecular complex (RON2/4/5/8), derived from secretory organelles called rhoptries, into the host cell membrane. AMA1, a protein secreted from micronemes and associated with the parasite surface during invasion, has been shown in vitro to bind the MJ complex through a direct association with RON2. Here we show that RON2 is inserted as an integral membrane protein in the host cell and, using several interaction assays with native or recombinant proteins, we define the region that binds AMA1. Our studies we

SUBMITTER: Lamarque M 

PROVIDER: S-EPMC3037350 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature