Comparative proteomics analysis of the root apoplasts of rice seedlings in response to hydrogen peroxide.

ABSTRACT:

Background

Plant apoplast is the prime site for signal perception and defense response, and of great importance in responding to environmental stresses. Hydrogen peroxide (H(2)O(2)) plays a pivotal role in determining the responsiveness of cells to stress. However, how the apoplast proteome changes under oxidative condition is largely unknown. In this study, we initiated a comparative proteomic analysis to explore H(2)O(2)-responsive proteins in the apoplast of rice seedling roots.

Methodology/principal findings

14-day-old rice seedlings were treated with low concentrations (300 and 600 µM) of H(2)O(2) for 6 h and the levels of relative electrolyte leakage, malondialdehyde and H(2)O(2) were assayed in roots. The modified vacuum infiltration method was used to extract apoplas

SUBMITTER: Zhou L 

PROVIDER: S-EPMC3037377 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature