Silk-based gene carriers with cell membrane destabilizing peptides.

ABSTRACT: Complexes of recombinant silk-polylysine molecules with ppTG1 peptide, a lysine-rich cell membrane destabilizing peptide to bind plasmid DNA (pDNA), are designed as less-cytotoxic and highly efficient gene carriers. The peptide destabilizes the cell membrane and promotes gene transfer. Our particular interest is in how ppTG1 enhances transfection efficiency of the silk-based delivery system into human cells. Genetically engineered silk proteins containing polylysine and the monomeric and dimeric ppTG1 sequences are synthesized in Escherichia coli , followed by transfection experiments. The pDNA complexes of silk-polylysine-ppTG1 dimer recombinant proteins prepared at an N/P 2 (the ratio of number of amines/phosphates from pDNA) shows the highest transfection efficiency into human embryonic

SUBMITTER: Numata K 

PROVIDER: S-EPMC3037451 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature