Three distinct modes of exocytosis revealed by amperometry in neuroendocrine cells.

ABSTRACT: Neurotransmission requires Ca(2+)-dependent release of secretory products through fusion pores that open and reclose (partial membrane distention) or open irreversibly (complete membrane distention). It has been challenging to distinguish between these release modes; however, in the work presented here, we were able to deduce different modes of depolarization-evoked exocytosis in neuroendocrine chromaffin and PC12 cells solely by analyzing amperometric recordings. After we determined the quantal size (Q), event half-width (t(50)), event amplitude (I(peak)), and event decay time constant (τ(decay)), we fitted scatter plots of log-transformed data with a mixture of one- and two-dimensional Gaussian distributions. Our analysis revealed three distinct and differently shaped clusters of secreto

SUBMITTER: van Kempen GT 

PROVIDER: S-EPMC3037570 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature