Gene set enrichment analysis of microarray data from Pimephales promelas (Rafinesque), a non-mammalian model organism.

ABSTRACT:

Background

Methods for gene-class testing, such as Gene Set Enrichment Analysis (GSEA), incorporate biological knowledge into the analysis and interpretation of microarray data by comparing gene expression patterns to pathways, systems and emergent phenotypes. However, to use GSEA to its full capability with non-mammalian model organisms, a microarray platform must be annotated with human gene symbols. Doing so enables the ability to relate a model organism's gene expression, in response to a given treatment, to potential human health consequences of that treatment. We enhanced the annotation of a microarray platform from a non-mammalian model organism, and then used the GSEA approach in a reanalysis of a study examining the biological significance of acute and chronic methylmercur

SUBMITTER: Thomas MA 

PROVIDER: S-EPMC3037904 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature