Evidence for mesenchymal-epithelial transition associated with mouse hepatic stem cell differentiation.

ABSTRACT:

Unlabelled

Mesenchymal-epithelial transition events are related to embryonic development, tissue construction, and wound healing. Stem cells are involved in all of these processes, at least in part. However, the direct evidence of mesenchymal-epithelial transition associated with stem cells is unclear. To determine whether mesenchymal-epithelial transition occurs in liver development and/or the differentiation process of hepatic stem cells in vitro, we analyzed a variety of murine liver tissues from embryonic day 11.5 to adults and the colonies derived from hepatic stem/progenitor cells isolated with flow cytometry. The results of gene expression, immunohistochemistry and Western blot showed that as liver develops, the expression of epithelial markers such as Cytokeratin18 and E-ca

SUBMITTER: Li B 

PROVIDER: S-EPMC3037942 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature