Characterization of alternative spliceoforms and the RNA splicing machinery in pancreatic cancer.

ABSTRACT:

Objectives and methods

Alternative splicing provides proteomic diversity that can have profound effects. The extent, pattern, and roles of alternative splicing in pancreatic cancer have not been systematically investigated. We have utilized a spliceoform-specific microarray and polymerase chain reaction to evaluate all known splice variants in human pancreatic cancer cell lines representing a spectrum of differentiation, from near-normal HPDE6 to Capan-1 and poorly differentiated MiaPaCa2 cells. Validation of altered spliceoforms was verified in primary cancer specimens and normal pancreatic ductal cells. In addition, expression of 92 spliceosomal genes was examined to better understand the mechanism for observed differences in mRNA splicing.

Results

A statistically signific

SUBMITTER: Carrigan PE 

PROVIDER: S-EPMC3038180 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature