Proteomic analysis of articular cartilage vesicles from normal and osteoarthritic cartilage.

ABSTRACT:

Objective

Articular cartilage vesicles (ACVs) are extracellular organelles found in normal articular cartilage. While they were initially defined by their ability to generate pathologic calcium crystals in cartilage of osteoarthritis (OA) patients, they can also alter the phenotype of normal chondrocytes through the transfer of RNA and protein. The purpose of this study was to analyze the proteome of ACVs from normal and OA human cartilage.

Methods

ACVs were isolated from cartilage samples from 10 normal controls and 10 OA patients. We identified the ACV proteomes using in-gel trypsin digestion, nanospray liquid chromatography tandem mass spectrometry analysis of tryptic peptides, followed by searching an appropriate subset of the Uniprot database. We further differentiated

SUBMITTER: Rosenthal AK 

PROVIDER: S-EPMC3038242 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature