MLL-AF9 and MLL-ENL alter the dynamic association of transcriptional regulators with genes critical for leukemia.

ABSTRACT:

Objective

The aim of this study was to better understand how mixed lineage leukemia (MLL) fusion proteins deregulate the expression of genes critical for leukemia.

Materials and methods

The transforming domain of one of the most common MLL fusion partners, AF9, was immunopurified after expression in myeloblastic M1 cells, and associating proteins were identified by mass spectrometric analysis. Chromatin immunoprecipitation followed by quantitative polymerase chain reaction was used to determine how binding of associating proteins compare across Hoxa9 and Meis1 in cell lines with and without MLL fusion proteins and how binding is altered during gene down-regulation and differentiation.

Results

Consistent with earlier purifications of ENL and AF4 from 293 cells, the 90

SUBMITTER: Monroe SC 

PROVIDER: S-EPMC3038424 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature