The pattern of somatic hypermutation of Ig genes is altered when p53 is inactivated.

ABSTRACT: Mice with a deletion of the p53 gene have normal antibody titers against sheep red blood cells and normal switching to all Ig isotypes. In older mice (11 and 16 weeks old) the somatic hypermutation (SHM) frequencies are progressively reduced. In young mice (8 weeks old) with p53 deletion, the SHM frequencies are normal. However, the mutation pattern is changed in all p53-/- mice: mutations at A are increased. Surprisingly, deletion of the Ung2 gene in addition to the deletion of p53 corrected the A mutation frequencies to those of control mice. Known interactions of p53 protein with several proteins involved in error-prone BER during SHM may explain these findings. There is no indication that the absence of p53 affects the function of AID. Inactivation of p21 does not alter SHM, supporting

SUBMITTER: Ratnam S 

PROVIDER: S-EPMC3038623 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature