Cloning approach and functional analysis of anti-intimin single-chain variable fragment (scFv).

ABSTRACT:

Background

Intimin is an important virulence factor involved in the pathogenesis of enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic Escherichia coli (EHEC). Both pathogens are still important causes of diarrhea in children and adults in many developing and industrialized countries. Considering the fact that antibodies are important tools in the detection of various pathogens, an anti-intimin IgG2b monoclonal antibody was previously raised in immunized mice with the conserved sequence of the intimin molecule (int388-667). In immunoblotting assays, this monoclonal antibody showed excellent specificity. Despite good performance, the monoclonal antibody failed to detect some EPEC and EHEC isolates harboring variant amino acids within the 338-667 regions of intimin molecu

SUBMITTER: Menezes MA 

PROVIDER: S-EPMC3038928 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature