Modeling RNA polymerase competition: the effect of σ-subunit knockout and heat shock on gene transcription level.

ABSTRACT:

Background

Modeling of a complex biological process can explain the results of experimental studies and help predict its characteristics. Among such processes is transcription in the presence of competing RNA polymerases. This process involves RNA polymerases collision followed by transcription termination.

Results

A mathematical and computer simulation model is developed to describe the competition of RNA polymerases during genes transcription on complementary DNA strands. E.g., in the barley Hordeum vulgare the polymerase competition occurs in the locus containing plastome genes psbA, rpl23, rpl2 and four bacterial type promoters. In heat shock experiments on isolated chloroplasts, a twofold decrease of psbA transcripts and even larger increase of rpl23-rpl2 transcripts we

SUBMITTER: Lyubetsky VA 

PROVIDER: S-EPMC3038987 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature