Understanding the effects of carbocyclic sugars constrained to north and south conformations on RNA nanodesign.

ABSTRACT: Relatively new types of the modified nucleotides, namely carbocyclic sugars that are constrained to north or south (C2' or C3' exo) conformations, can be used for RNA nanoparticle design to control their structures and stability by rigidifying nucleotides and altering the helical properties of RNA duplexes. Two RNA structures, an RNA dodecamer and an HIV kissing loop complex where several nucleotides were replaced with north or south constrained sugars, were studied by molecular dynamics (MD) simulations. The substituted south constrained nucleotides in the dodecamer widened the major groove and narrowed and deepened the minor groove thus inducing local conformational changes that resemble a B-form DNA helix. In the HIV kissing loop complex, north and south constrained nucleotides were sub

SUBMITTER: Kim T 

PROVIDER: S-EPMC3040123 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature