Pooled genome-wide analysis to identify novel risk loci for pediatric allergic asthma.

ABSTRACT:

Background

Genome-wide association studies of pooled DNA samples were shown to be a valuable tool to identify candidate SNPs associated to a phenotype. No such study was up to now applied to childhood allergic asthma, even if the very high complexity of asthma genetics is an appropriate field to explore the potential of pooled GWAS approach.

Methodology/principal findings

We performed a pooled GWAS and individual genotyping in 269 children with allergic respiratory diseases comparing allergic children with and without asthma. We used a modular approach to identify the most significant loci associated with asthma by combining silhouette statistics and physical distance method with cluster-adapted thresholding. We found 97% concordance between pooled GWAS and individual genoty

SUBMITTER: Ricci G 

PROVIDER: S-EPMC3040188 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature