Assessing protein immunogenicity with a dendritic cell line-derived endolysosomal degradome.

ABSTRACT:

Background

The growing number of novel candidate molecules for the treatment of allergic diseases imposed a dramatic increase in the demand for animal experiments to select immunogenic vaccines, a pre-requisite for efficacy. Because no in vitro methods to predict the immunogenicity of a protein are currently available, we developed an in vitro assay that exploits the link between a protein's immunogenicity and its susceptibility to endolysosomal proteolysis.

Methodology

We compared protein composition and proteolytic activity of endolysosomal fractions isolated from murine bone marrow- and human blood- derived dendritic cells, and from the dendritic cell line JAWS II. Three groups of structurally related antigen variants differing in their ability to elicit immune responses

SUBMITTER: Egger M 

PROVIDER: S-EPMC3040223 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature