Structural NMR of protein oligomers using hybrid methods.

ABSTRACT: Solving structures of native oligomeric protein complexes using traditional high-resolution NMR techniques remains challenging. However, increased utilization of computational platforms, and integration of information from less traditional NMR techniques with data from other complementary biophysical methods, promises to extend the boundary of NMR-applicable targets. This article reviews several of the techniques capable of providing less traditional and complementary structural information. In particular, the use of orientational constraints coming from residual dipolar couplings and residual chemical shift anisotropy offsets are shown to simplify the construction of models for oligomeric complexes, especially in cases of weak homo-dimers. Combining this orientational information with int

SUBMITTER: Wang X 

PROVIDER: S-EPMC3040251 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature