The maternal and early embryonic transcriptome of the milkweed bug Oncopeltus fasciatus.

ABSTRACT:

Background

Most evolutionary developmental biology ("evo-devo") studies of emerging model organisms focus on small numbers of candidate genes cloned individually using degenerate PCR. However, newly available sequencing technologies such as 454 pyrosequencing have recently begun to allow for massive gene discovery in animals without sequenced genomes. Within insects, although large volumes of sequence data are available for holometabolous insects, developmental studies of basally branching hemimetabolous insects typically suffer from low rates of gene discovery.

Results

We used 454 pyrosequencing to sequence over 500 million bases of cDNA from the ovaries and embryos of the milkweed bug Oncopeltus fasciatus, which lacks a sequenced genome. This indirectly developing insect o

SUBMITTER: Ewen-Campen B 

PROVIDER: S-EPMC3040728 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature