Quantitative 3-dimensional imaging of murine neointimal and atherosclerotic lesions by optical projection tomography.

ABSTRACT:

Objective

Traditional methods for the analysis of vascular lesion formation are labour intensive to perform - restricting study to 'snapshots' within each vessel. This study was undertaken to determine the suitability of optical projection tomographic (OPT) imaging for the 3-dimensional representation and quantification of intimal lesions in mouse arteries.

Methods and results

Vascular injury was induced by wire-insertion or ligation of the mouse femoral artery or administration of an atherogenic diet to apoE-deficient mice. Lesion formation was examined by OPT imaging of autofluorescent emission. Lesions could be clearly identified and distinguished from the underlying vascular wall. Planimetric measurements of lesion area correlated well with those made from histological s

SUBMITTER: Kirkby NS 

PROVIDER: S-EPMC3040742 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature