Rho-kinase signalling regulates trypsinogen activation and tissue damage in severe acute pancreatitis.

ABSTRACT:

Background and purpose

Severe acute pancreatitis (SAP) is characterized by trypsinogen activation, infiltration of leucocytes and tissue necrosis but the intracellular signalling mechanisms regulating organ injury in the pancreas remain elusive. Rho-kinase is a potent regulator of specific cellular processes effecting several pro-inflammatory activities. Herein, we examined the role of Rho-kinase signalling in acute pancreatitis.

Experimental approach

Pancreatitis was induced by infusion of taurocholate into the pancreatic duct in C57BL/6 mice. Animals were treated with a Rho-kinase inhibitor Y-27632 (0.5-5 mg·kg⁻¹) before induction of pancreatitis.

Key results

Taurocholate infusion caused a clear-cut increase in blood amylase, pancreatic neutrophil infiltration, acin

SUBMITTER: Awla D 

PROVIDER: S-EPMC3041254 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature