Multiplex target enrichment using DNA indexing for ultra-high throughput SNP detection.

ABSTRACT: Screening large numbers of target regions in multiple DNA samples for sequence variation is an important application of next-generation sequencing but an efficient method to enrich the samples in parallel has yet to be reported. We describe an advanced method that combines DNA samples using indexes or barcodes prior to target enrichment to facilitate this type of experiment. Sequencing libraries for multiple individual DNA samples, each incorporating a unique 6-bp index, are combined in equal quantities, enriched using a single in-solution target enrichment assay and sequenced in a single reaction. Sequence reads are parsed based on the index, allowing sequence analysis of individual samples. We show that the use of indexed samples does not impact on the efficiency of the enrichment reacti

SUBMITTER: Kenny EM 

PROVIDER: S-EPMC3041504 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature