Bacillus pumilus laccase: a heat stable enzyme with a wide substrate spectrum.

ABSTRACT:

Background

Laccases are multi-copper oxidases that catalyze the one electron oxidation of a broad range of compounds. Laccase substrates include substituted phenols, arylamines and aromatic thiols. Such compounds are activated by the enzyme to the corresponding radicals. Owing to their broad substrate range laccases are considered to be versatile biocatalysts which are capable of oxidizing natural and non-natural industrial compounds, with water as sole by-product.

Results

A novel CotA-type laccase from Bacillus pumilus was cloned, expressed and purified and its biochemical characteristics are presented here. The molecular weight of the purified laccase was estimated to be 58 kDa and the enzyme was found to be associated with four copper atoms. Its catalytic activity towards

SUBMITTER: Reiss R 

PROVIDER: S-EPMC3041658 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature