Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay.

ABSTRACT:

Background

Protein interactions control the regulatory networks underlying developmental processes. The understanding of developmental complexity will, therefore, require the characterization of protein interactions within their proper environment. The bimolecular fluorescence complementation (BiFC) technology offers this possibility as it enables the direct visualization of protein interactions in living cells. However, its potential has rarely been applied in embryos of animal model organisms and was only performed under transient protein expression levels.

Results

Using a Hox protein partnership as a test case, we investigated the suitability of BiFC for the study of protein interactions in the living Drosophila embryo. Importantly, all BiFC parameters were established wi

SUBMITTER: Hudry B 

PROVIDER: S-EPMC3041725 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature