Comprehensive analysis of alternative splicing and functionality in neuronal differentiation of P19 cells.

ABSTRACT:

Background

Alternative splicing, which produces multiple mRNAs from a single gene, occurs in most human genes and contributes to protein diversity. Many alternative isoforms are expressed in a spatio-temporal manner, and function in diverse processes, including in the neural system.

Methodology/principal findings

The purpose of the present study was to comprehensively investigate neural-splicing using P19 cells. GeneChip Exon Array analysis was performed using total RNAs purified from cells during neuronal cell differentiation. To efficiently and readily extract the alternative exon candidates, 9 filtering conditions were prepared, yielding 262 candidate exons (236 genes). Semiquantitative RT-PCR results in 30 randomly selected candidates suggested that 87% of the candidates

SUBMITTER: Suzuki H 

PROVIDER: S-EPMC3041816 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature