Purine receptor-mediated endocannabinoid production and retrograde synaptic signalling in the cerebellar cortex.

ABSTRACT:

Background and purpose

Presynaptic CB₁ cannabinoid receptors can be activated by endogenous cannabinoids (endocannabinoids) synthesized by postsynaptic neurones. The hypothesis of the present work was that activation of calcium-permeable transmitter-gated ion channels in postsynaptic neurones, specifically of P2X purine receptors, can lead to endocannabinoid production and retrograde synaptic signalling.

Experimental approach

GABAergic inhibitory postsynaptic currents (IPSCs) were recorded with patch-clamp techniques in Purkinje cells in mouse cerebellar slices. Purine receptors on Purkinje cells were activated by pressure ejection of ATP from a pipette.

Key results

ATP evoked an inward current in Purkinje cells, most likely due to P2X receptor activation. The ATP-evo

SUBMITTER: Kovacs FE 

PROVIDER: S-EPMC3042206 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature